Berita

Seminar Nasional:
Bioetika Pertanian 2008

Bogor,29 Mei 2008

 

Sequence Analysis of 2b Gene of Australian CMV Isolates

Emy Sulistyowati
Indonesian Research Institute for Tobacco and Fiber Crops, Malang

ABSTRAK

The Cucumber mosaic virus (CMV) 2b gene has been reported to function as a suppressor of gene silencing, as a systemic movement determinant, and as a host-specific hypervirulence determinant. Seven Australian CMV isolates has been used for this study, from which their 2b genes were sequenced and analysed. The subgrouping of 7 Australian CMV isolates has been deduced by analysing the 2b gene sequences, the protein sequences, and the size of the 2b ORF. The 7 CMV isolates used in this study are members of different subgroups i.e. subgroup Ia (CMV 207, CMV 237, CMV 242, and Twa CMV), subgroup Ib (CMV 243), and subgroup II (CMV 241 and CMV 245). Moreover, the 2b genes of Australian CMV isolates within subgroups IA and II are conserved in size and sequence and contain a nuclear localisation signal (NLS).

Kata kunci: -

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